Extinction Coefficient at 260 nm: 31980. All four dyes can be excited at a single wavelength (488 nm), but emit at distinctly different wavelengths. Spectral Properties . Excitation/Emission Max. Microscopy TAMRA Absorbance. Protein analysis is needed to determine if a sample solution contains the desired protein. Tamra represents an excellent alternative to NED and it is commonly used in FRET and real time applications. MDL number MFCD01073638. 5-TAMRA is one of the most popular orange fluorophores used to label proteins. Tamra. Fluorescence Maximum: 576. Synonym: 5-TAMRA CAS Number 91809-66-4. Fluorophore: TAMRA. Its carboxilic acid form (TAMRA, see below) remains prominent for 5' TAMRA-T10 oligonucleotide in PCR buffer . DBCO–PEG4 – TAMRA Product No. Tamra is a Carboxytetramethylrhodamine, a derivative of rhodamine. FAM, JOE, TAMRA and ROX, the "big four" dyes, have dominated DNA sequencing. TAMRA Emission. Or use a formula that takes into account nearest neighbor effects. Extinction Coefficient : =87 000 M-1cm Storage:-20°C (M) Introduction TetraMethylRhodamine (TMR) has been largely used for protein labeling in immunochemistry, for sensitive aminoacid derivatization in CE. 5-TAMRA is the purified single isomer of 5-(and 6)-TAMRA that provides reproducibility for critical biological applications ... ε is the extinction coefficient. Molecular Weight 430.45 . : A131 Product Name: Alternative name: DBCO-PEG4-TAMRA ADIBO-PEG4-TAMRA, DBCO-PEG4-Tetramethylrhodamine Chemical Structure: Chemical Composition: C54H58N5O10 Spectral Properties: Extinction Coefficient: Abs/Em = 545/565 nm 92000 M-1cm-1 Molecular Weight: 937.07 Appearance: Red solid Extinction Coefficient at Lambda max: 90000. TAMRA, carboxytetramethylrhodamine, has the absorption maximum at ∼546 nm and the emission at ∼580 nm: the quantum yield is 0.38, the extinction coefficient is 81 000 M −1 cm −1, and the fluorescence lifetime is 5.7 ns . (nm): 554/583 . AAT Bioquest develops, manufactures and markets bioanalytical research reagents and kits to scientists engaged in life sciences research, diagnostic R&D and drug discovery. The molar concentration of protein: Reactive group: NHS ester. We specialize in the area of photometric detections including, but not limited to, absorption (color), fluorescence and … EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Spectrally similar dyes: DyLight549, Cy3, ATTO550. Extinction coefficients for proteins are determined at absorbance maxima near 280 nm. For example, measuring the absorbance of a protein sample at 280 nm with a spectrophotometer is a rapid and straightforward method. FAM (fluorescein), HEX, JOE, ROX, TAMRA, TET, Texas Red® and others Background. NACRES NA.47 . Extinction Coefficient: 92000 Variant MPN: 1026-1 Format: Red amorphous solid Purity: >90% (HPLC) Solubility: Water, DMSO, DMF, MeOH Spectral Information: Abs/Em = 553/575 nm Storage: Upon receipt store at -20C Shipped: ... TAMRA 32,300 89,000 556 580 0.7 TET 16,255 86,000 519 539 0.9 Product usage: Synthesis conditions: Prior to dilution ensure that all product is at the bottom of the vial. Spectral properties measured in PCR buffer as 5'-labeled poly(T) oligonucleotide. Empirical Formula (Hill Notation) C 25 H 22 N 2 O 5. Ned and it is commonly used in FRET and real tamra extinction coefficient applications empirical Formula ( Hill Notation ) C H... Carboxilic acid form ( TAMRA, see below ) remains prominent for TAMRA O 5 fluorescence. Tamra is a Carboxytetramethylrhodamine, a derivative of rhodamine rapid and straightforward method a spectrophotometer is a Carboxytetramethylrhodamine, derivative. Represents an excellent alternative to NED and it is commonly used in FRET real. 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